In order to homogenize the samples, isomerized pellets are ground prior to commencing the analysis.
Isomerized pellets intended for use in beer brewing or elsewhere in the food industry
The isomerized pellets are placed in a suitable mill, such as a coffee mill, are then ground to the point that they are sufficiently crushed to be analyzed.
Isomerized pellets intended for use in beer brewing or elsewhere in the food industry
The reliable sample collection from hop products, isomerized pellets in this instance, is the prerequisite for obtaining suitable material for analysis.
This method describes how to determine iso-α-acids, α-acids and β-acids in isomerized pellets by means of reverse phase high pressure liquid chromatography (RP-HPLC).
Isomerized pellets intended for use in beer brewing or elsewhere in the food industry
The bitter substances in isomerized hop pellets contain a substantial amount of iso-α-acids; however, in addition to these, non-isomerized α-acids and β-acids are also present. In order to determine their content, a specific method is required.
After milling, the substances in question are extracted from the isomerized pellets using a diethyl ether/methanol mixture and a hydrochloric acid solution. The iso-α-acids, α-acids and β-acids dissolved in the ether phase are separated using reversed-phase high-performance liquid chromatography (RP-HPLC) and an elution gradient. They are then measured spectrophotometrically at wavelengths of 270 nm (iso-α-acids) and 314 nm (α-acids and β-acids).
Isomerized hop extract intended for use in beer brewing or elsewhere in the food industry
A representative sample of homogenized material is necessary for the analysis. For this reason, if at all possible, samples of the hops or hop products should be taken from their original packaging for analysis.
The method describes how to determine the iso-α-acids, α-acids and β-acids in hop extracts and isomerized extracts by means of high-pressure liquid chromatography.
Hop extract and isomerized hop extract intended for use in beer brewing or elsewhere in the food industry
Hop extracts and isomerized hop extracts are dissolved in methanol. The iso-α-acids, α-acids and β-acids are separated using hig-pressure liquid chromatography (HPLC) with gradient elution and measured spectrophotometrically at a wavelength of 270 nm (iso-α-acids) and 314 nm (α-acids and β-acids).
Isomerized hop extract intended for use in beer brewing or elsewhere in the food industry
A representative sample of the hop extract is necessary for the analysis, i.e., the sample should be taken from a completely homogenized container.