Hops and hop products intended for use in beer brewing or elsewhere in the food industry
A reliable sampling method for whole hops and hop products is a prerequisite for obtaining suitable material for analysis. The effort involved in collecting the sample depends upon how uniform the hop product is.
This method describes the determination of four hop aroma compounds found in hops and hop products using gas chromatography.
Hops and hop products intended for use in beer brewing or elsewhere in the food industry
The hop oil obtained through steam distillation (refer to links) is dissolved in an organic solvent, separated into its components by means of gas chromatography and determined with a flame ionization detector. The contents are expressed as a percentage of the area of each component compared to the overall area of the peak.
Whole hops intended for use in beer brewing or elsewhere in the food industry
Evaluation of the appearance of hop cones is performed through visual inspection and sensory assessment.
Hops and hop products intended for use in beer brewing or elsewhere in the food industry
After extraction with toluene, the α-acids and β-acids in the hops and hop pellets are determined using spectrophotometry.
Hops and hop products intended for use in beer brewing or elsewhere in the food industry
After milling, hops and hop powder products are extracted using a diethyl ether/methanol mixture and a hydrochloric acid solution. The α-acids and β-acids dissolved in the ether phase are separated using reversed phase high-pressure liquid chromatography (RP-HPLC) and measured spectrophotometrically at a wavelength of 314 nm.
Hop extracts are dissolved in methanol. The α-acids and β-acids dissolved in the methanol are separated using reversed phase high pressure liquid chromatography (RP-HPLC) and measured spectrophotometrically at a wavelength of 314 nm.
Hops and hop products intended for use in beer brewing or elsewhere in the food industry
The volatile constituents in hops or hop products are obtained by means of steam distillation. The hydrocarbon and oxygen fractions are separated using a process involving column chromatography.