R-300.08.151 [2016-03] Myrcene, Beta-Caryophyllene, Beta-Farnesene and Alpha-Humulene in Whole Hops and Hop Products – GC

The aroma compounds in hops are also referred to as (essential) hop oil. The determination of the content of these aroma compounds in hops allows those employing hops or hop products to dose them accordingly on the basis of their aroma. 

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Application/Purpose

This method describes the determination of four hop aroma compounds found in hops and hop products using gas chromatography.

Scope of Application

Hops and hop products intended for use in beer brewing or elsewhere in the food industry

Principle

The hop oil obtained through steam distillation (refer to links) is dissolved in an organic solvent, separated into its components by means of gas chromatography and determined with a flame ionization detector. The contents are expressed as a percentage of the area of each component compared to the overall area of the peak.

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