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B-590.49.133 [2020-10] Vitamin B2 – HPLC

Numerous beverages, such as multivitamin juice and sports drinks, are enriched with vitamins. These beverages may not be listed on a label as "enriched" or "fortified" with a particular vitamin supplement unless the vitamin is present in "significant amounts", which are defined in Annex XIII to Regulation (EU) No 1169/2011. For beverages, this amount is set at a minimum of 7.5 % of the NRV (nutrient reference values) per 100 ml or 15 % of the NRV per portion for individual portions.

Quantities recommended for the daily dietary intake of vitamins:

vitamin B₁ (thiamine)	1.1	mg
vitamin B₂(riboflavin)	1.4	mg
vitamin B₆ (pyridoxine)	1.4	mg
pantothenic acid	6	mg
folic acid	200	µg
niacin	16	mg
vitamin B₁₂	2.5	µg
ascorbic acid	80	mg
vitamin E	12	mg
biotin	50	µg
vitamin A*	800	µg

* 1 µg vitamin A corresponds to 6 µg all-trans-β-carotene or 12 µg of other provitamin-A carotenoids.

Approx. 50 carotenoids exhibit a provitamin-A effect. Within the confines of a normal diet, however, only α-carotene, β-carotene and β-cryptoxanthin are of any significance.

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vitamins

vitamin B2

riboflavin

riboflavin-5'-phosphate

juice

NAB

beverage base

Application/Purpose

Analysis for determining the content of added vitamin B₂ using HPLC

Scope of Application

This analysis is suitable for NAB, juice, beverage bases and vitamin powder.

Principle

Vitamin B₂, riboflavin, and riboflavin-5'-phosphate are separated using reversed-phase HPLC and detected with a fluorescence detector.