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B-590.47.131 [2020-10] Niacin – HPLC

Numerous beverages, such as multivitamin juice and sports drinks, are enriched with vitamins. These beverages may not be listed on a label as "enriched" or "fortified" with a particular vitamin supplement unless the vitamin is present in "significant amounts", which are defined in Annex XIII to Regulation (EU) No 1169/2011. For beverages, this amount is set at a minimum of 7.5 % of the NRV (nutrient reference values) per 100 ml or 15 % of the NRV per portion for individual portions.

Quantities recommended for the daily dietary intake of vitamins:

vitamin B₁ (thiamine)	1.1	mg
vitamin B₂(riboflavin)	1.4	mg
vitamin B₆ (pyridoxine)	1.4	mg
pantothenic acid	6	mg
folic acid	200	µg
niacin	16	mg
vitamin B₁₂	2.5	µg
ascorbic acid	80	mg
vitamin E	12	mg
biotin	50	µg
vitamin A*	800	µg

* 1 µg vitamin A corresponds to 6 µg all-trans-β-carotene or 12 µg of other provitamin-A carotenoids.

Approx. 50 carotenoids exhibit a provitamin-A effect. Within the confines of a normal diet, however, only α-carotene, β-carotene and β-cryptoxanthin are of any significance.

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Application/Purpose

Analysis for determining the content of added niacin using HPLC

Scope of Application

This analysis is suitable for NAB, juice, beverage bases and vitamin powder.

Principle

Niacin is separated by ion-pair RP-HPLC, detected using UV absorption and quantified through comparison with external standards. If necessary, the identity of the substances can be confirmed via spectra comparison.