Determination of the soluble extract in wet spent grain obtained by pressing
Wet spent grain
Wet spent grain is pressed using a manual press.
Determination of the soluble extract in the spent grain through rinsing
Wet spent grain, dry spent grain
The soluble extract in the spent grain is determined by rinsing with H2O at 70 °C.
Determination of the total extract in the spent grain through enzymatic digestion
Wet spent grain, dry spent grain
The total extract is obtained through boiling the spent grain followed by enzymatic digestion with technical enzymes (Termamyl 120 L and SAN Super 360 L). The available residual extract represents the difference between total extract and soluble extract.
Determination of the total extract in the spent grain using the diastase method
Wet spent grain, dry spent grain
The total extract is obtained through boiling the spent grain followed by enzymatic digestion with diastase obtained from malt. The available residual extract represents the difference between total extract and soluble extract.
Suitable for Congress mash
Wort is produced according to the Congress mash method (fine grind), and then the nitrogen content of the wort is determined.
Suitable for all (laboratory) worts
Wort is produced using a laboratory mash method (fine grind) after which the nitrogen content of the wort is determined.