This method describes how to determine the sulfate content in water by cation exchange.
A water sample is run through an ion exchanger in which all of the cations are replaced with hydrogen ions. The sulfate is determined through titration in the presence of a previously prepared barium chloride solution, of which a known amount in excess of that required is added in advance. The quantity is measured by complexometric titration. The difference between the initial concentration of barium chloride and the amount determined by back titration corresponds to the sulfate content.
In many cases, particularly at higher contents, an alternative and sufficiently accurate measurement is possible, called the “negative m value” or the “total mineral acid value” (without carbonic acid). This is achieved through titration. Subsequently, the mval values for the anions (Cl-, NO3-, NO2-, PO43-) are subtracted from the result.
Whole hops intended for use in beer brewing or elsewhere in the food industry
Evaluation of the appearance of hop cones is performed through visual inspection and sensory assessment.
Malt intended for use in beer brewing or elsewhere in the food industry.
This method is based upon on the fact that the β-glucan-rich cell walls of the endosperm are progressively broken down during malting. This process can be made visible by staining the cell walls that are still intact with the fluorochrome Calcofluor, which exclusively binds β-glucans starting at a molecular weight of approx. 10,000 D.
Modification is revealed by allowing the barley kernels, which have been cut in half, to react with Calcofluor (with Fast Green as a contrast medium). The kernels are subsequently examined under UV light (365 nm) in a suitable analyzer device. An intense, bright blue fluorescence occurs where unmodified endosperm cells are present, while the modified parts appear dark blue.
This method describes how to determine the organic acids in wort and the Congress wort using a cation exchanger.
Applicable for all (laboratory) worts
A cation exchanger based upon a sulfonated, crosslinked styrene/divinylbenzene copolymer is used to determine various organic acids in wort. Due to the high ligand density, the separation mechanism is based upon a combination of ion exclusion, ligand exclusion and steric exclusion; detection is performed using a UV detector.
This method describes how to perform the iodine test and measure the saccharification time in the Congress mash method.
Malt intended for use in beer brewing or elsewhere in the food industry
The iodine test is performed to determine the time required for saccharification during mashing. It is to be evaluated based on the type of malt used for mashing.
Determination of the percentage of nitrogen that has dissolved in the wort during the Congress mash method
Suitable for (Congress) mash
The Kolbach index is used to express the quantity of the nitrogenous substances found in malt, which go into solution under the conditions present during the Congress mash method. It is a measure of the degree of proteolytic modification of the malt and also provides an indication for the quantity of proteolytic enzymes contained in the malt. The Kolbach index is less conclusive than other methods due to its dependence on the total nitrogen content and the provenance of the barley. Therefore, it must always be considered together with the total nitrogen content.