Peracetic acid (C2H4O5) not only reacts with the proteins of the cell wall of microorganisms, but also penetrates into the cell interior as a slightly dissociated acid. It has an oxidative-destructive effect here and there, i.e. non-specifically on all protein components of the cell and therefore also on any enzyme systems. As a result, these enzymes are destroyed and the cell is killed.
Determination of peracetic acid (C2H4O5) in disinfectants.
Suitable for all solutions containing peracetic acid (C2H4O5).
In addition to peracetic acid (C2H4O5), hydrogen peroxide (H2O2) is always present in disinfectants, so that both components must be determined together. The quantitative determination of hydrogen peroxide is carried out as described under T-753.01.032 Disinfectants containing hydrogen peroxide - potassium permanganate method or T-753.02.032 Disinfectants containing hydrogen peroxide - sodium thiosulphate method. The peracetic acid is determined iodometrically:
|
H2O2 + 2 NaI +H2SO4 |
→ |
I2 + Na2SO4 + 2 H2O |
|
I2 + Na2S2O3 |
→ |
2 NaI + Na2S2O3 |