T-753.02.032 [2024-08] Disinfectants containing hydrogen peroxide - sodium thiosulphate method

The disinfecting effect of hydrogen peroxide systems from its oxidative action which irreversibly destroys and kills the biological active systems of microorganisms.

 

Application/Purpose

Determination of hydrogen peroxide (H2O2) in disinfectants.

Scope of Application

Suitable for all solutions that contain hydrogen peroxide (H2O2) but no other peroxides.

Principle

Hydrogen peroxide oxidizes iodide to iodine, which is reduced by sodium thiosulfate (Na2S2O3) and recalculated as hydrogen peroxide.

H2O2 + 2 NaI +H2SO4

  I2 + Na2SO4 + 2 H2O

I2 + Na2S2O3

2 NaI + Na2S2O3

If the sample contains other peroxide-containing disinfectants in addition to hydrogen peroxide (H2O2), the method T-754.01.032 Peracetic acid-based disinfectants must be used.

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