The method is suitable for beers of all original gravities and of any alcohol content.
The gas chromatography headspace method is used to determine the higher alcohols and esters present in beer, i.e., the volatile compounds are transferred from the headspace in the sample vial into the GC system for analysis. The following substances are measured in this analysis:
Acetaldehyde
Propanol-1
Ethyl acetate
2-Methylpropanol
3-Methylbutanol
2-Methylbutanol
2-Methylpropylacetate
Butyric acid ethyl ester
3-Methylbutyl acetate
2-Methylbutyl acetate
Hexanoic acid ethyl ester
Applicable for all (laboratory) worts
The Congress wort is heated in order to inactivate the amylolytic enzymes, and afterwards, yeast is added and the wort is allowed to completely ferment out at a minimum temperature of 20 °C in a fermentation tube (fig. 1). The difference in the extract before and after fermentation is measured in order to calculate the limit of attenuation.
Applicable for all (laboratory) wort
The Congress wort is heated in order to inactivate the amylolytic enzymes, and afterwards added to an Erlenmeyer flask along with 7.5 g/100 ml yeast where it is allowed to completely ferment out under constant agitation. The difference in the extract before and after fermentation is measured in order to calculate the limit of attenuation.
The method is suitable for beers of all original wort ranges and alcohol contents.
Volatile compounds in beer are concentrated through distillation and the distillate is quantitatively determined by direct injection into a gas chromatograph. The linearity of the detector and the determination of the concentrations of analytes in the sample are achieved by using multiple concentration levels within the relevant range and through evaluation of the relative area under the peaks.
This method is suitable for beers exhibiting all ranges of original gravity and alcohol concentrations.
The method is suitable for beer brewed to any original gravity or to any alcohol content.
Volatile compounds in beer are concentrated through distillation and extracted with dichloromethane. The solvent phase is analyzed with a gas chromatograph. The linearity of the detector and the determination of the concentrations of analytes in the sample are achieved by using multiple concentration levels within the relevant range and through evaluation of the relative area under the peaks.
Determination of 4-vinylguaiacol and 4-vinylphenol in beer
The method is suitable for beer brewed to all ranges of original gravity and alcohol content.
Analysis was performed using isocratic HPLC on a Spherisorb separation column with UV detection at 260 nm.