Whole hops intended for use in beer brewing or elsewhere in the food industry
Evaluation of the appearance of hop cones is performed through visual inspection and sensory assessment.
Determination of xanthohumol and isoxanthohumol
All beers, beer-based beverages, wort, ethanol extracts, CO2 spent hops and xanthohumol products
Xanthohumol and isoxanthohumol are dissolved with acetonitrile from the sample and following separation, are determined using a Nucleodur C18 column and UV detection.
Spectrophotometric determination of the iodine value of brewery spent grain
Brewery spent grain, wet spent grain, dry spent grain
High molecular weight dextrins and starch present in the wort extracted from brewery spent grain are precipitated through the addition of ethanol, centrifuged and dissolved in phosphate buffer, followed by the addition of an iodine solution. Depending on the molecular weight and degree of branching, a red to blue color forms, the intensity of which is measured spectrophotometrically at 578 nm.
Hops and hop products intended for use in beer brewing or elsewhere in the food industry
The respective constituents of hops are not uniformly distributed throughout the cones. The aroma compounds and bitter substances are found in the lupulin glands inside the cone, adhering to the cone bracteoles near the strig. The polyphenols, on the other hand, are found in the bracts, bracteoles and stems. In order to reliably analyze the constituents of hop cones, homogenization of the sample is required. For this purpose, the cones are ground and the sample is divided.
Hops with high moisture content have a shorter storage life; in contrast, hops that are too dry disintegrate easily and lose their lupulin.
Hops and hop products intended for use in beer brewing or elsewhere in the food industry
The moisture content of whole hops, hop powder products or pellets is determined through the difference in weight before and after drying under standardized conditions.
Hops and hop products intended for use in beer brewing or elsewhere in the food industry
Hop constituents are distributed between an aqueous acidic methanolic phase and diethyl ether. Hop bitter substances extracted with ether are subsequently separated according to their different solubility properties in cold methanol and hexane into fractions: total resins, soft resins and hard resins. The soft resins are further separated according to their capacity to form complexes with lead salts into α-acids (conductometer value) and a β-fraction.