The wort analysis methods are written, for example, for wort that is produced using the Congress mash method. These methods may also be applied in the analysis of wort which has been produced using a different mash method, particularly the isothermal 65 °C method. However, the reference values listed in the analysis method for Congress wort are not transferable.
This method describes how to determine the fermentable carbohydrates in wort or in the Congress wort using high performance liquid chromatography.
Applicable for all (laboratory) worts
Fructose, glucose as well as disaccharides and trisaccharides (maltose and maltotriose) are determined using high performance liquid chromatography (HPLC).
Wort or beer is deionized using an ion exchanger. The sample is filtered and concentrated using a solid-phase column and analyzed chromatographically. The concentration of the sugars present in the sample is calculated from the chromatograms obtained by analysis of the calibration solutions.