The wort analysis methods are written, for example, for wort that is produced using the Congress mash method. These methods may also be applied in the analysis of wort which has been produced using a different mash method, particularly the isothermal 65 °C method. However, the reference values listed in the analysis method for Congress wort are not transferable.
The method describes how to determine the β-glucans in wort or Congress wort by means of a colorimetric method.
Applicable for all (laboratory) worts
The colorimetric method may be used for the determination of high molecular weight β-glucan in wort; it is based on the photometric evaluation of a β-glucan color complex. The soluble β-glucan from barley reacts with a buffered solution, forming a color complex within 30 minutes. The absorbance of the β-glucan color complex is measured at 550 nm.