B-420.19.151 [2020-10] Aromatic Alcohols

Aromatic alcohols are compounds which exhibit a strong sensory influence on beer. This is especially true of some top-fermented beers with the pronounced aroma of guaiacol. Phenolcarboxylic acids are precursors of these substances and, in fact, possess antioxidative properties.

4-Vinylguaiacol and 4-vinylphenol are derivatives of ferulic acid and coumaric acid, respectively. Both substances appear in higher concentrations in Southern German wheat beers (Weissbier). 4-Vinyl guaiacol is considered an indicator substance.

4-Vinylguaiacol and 4-vinylphenol are compounds that play an important role in beer, especially in wheat beer and pale lager. These compounds are produced exclusively by POF-positive yeasts (phenol-off-flavor). While 4-vinylguaiacol is desirable in wheat beer and provides the characteristic spicy and clove aromas, both compounds are undesirable in pale lager beer and are considered off-flavors. These off-flavors can be caused by contamination with foreign yeasts and various lactobacilli, which has a negative impact on the quality and taste of the beer.

Application/Purpose

Determination of the aromatic alcohols guaiacol, tryptophol, 4-ethyl guaiacol, 4-vinyl guaiacol, eugenol, tyrosol, 4-ethylphenol, 2-phenylethanol in beer

Scope of Application

The method is suitable for beer brewed to any original gravity or to any alcohol content.

Principle

The aromatic alcohols in beer are isolated using solid-phase extraction and are detected using gas chromatography-mass spectrometry.

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